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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP465953BYTYeast1mgQuote
EP465953BYTE. coli1mgQuote
BP465953BYTBaculovirus200ugQuote
MP465953BYTMammalian Cell200ugQuote

Protein Information

SpeciesAnaeromyxobacter sp. (strain K)
UniProt IDB4UAN1
Gene NameleuD; Locus:AnaeK_1899
Protein Name
Region Expressed1-191
Expression Tag6xHis
Purity>90%
AA SequenceMEPIRVIESRTVVLPRENVDTDQIIPARFLKVTDKKGLGKALFSDWRYAADGAPRPDFVM NRPEAQGCSILVAGDNFGCGSSREHAPWALVDAGVRAVISTRIADIFRNNALKNGLVPVV LDAASHAKLLAAPGASVRVDVEAQTVTLPDGSTARFPLDGFARYCLLNGVDELGFLLAQE ADIAAFEGGRR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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