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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP480339HXTYeast1mgQuote
EP480339HXTE. coli1mgQuote
BP480339HXTBaculovirus200ugQuote
MP480339HXTMammalian Cell200ugQuote

Protein Information

SpeciesHydrogenobaculum sp. (strain Y04AAS1)
UniProt IDB4U7U5
Gene NameleuC; Locus:HY04AAS1_0519
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-424
Expression Tag6xHis
Purity>90%
AA SequenceMGHTITEKIIADHAGKKEVFPGELVTAKIDLAMANDVTAPLSIKTLEKYGIEKVFDKDKI ALVLSHFVPAKDIKSAEQAKIVRDFAKKHNIKWFFGEGEGIEHTLLPENGIVVPGDLVVG ADSHTCTYGGIGAFSTGVGSTDLAYAMATGEIWLKVPESMKFIFYGKLNKWVSGKDLILY TIGQIGVDGALYRAMEFDGEAIRSLDVSQRLTIANMAIEAGGKSGIISPDEKTIEYVEKR AKKPYKIYQSDKDAHYVEVYEWDASSIEPMVAWPYLPSNVHPVSESTHITIDQAFIGSCT NGRIEDLRIAASILKGKKVHPYTRCVVIPASKNVYLQALHEGLVDIFIEAGCAVSTSTCG PCLGGHMGILAKGERCISTSNRNFPGRMGHPQSEAYLANPAVVAASAVLGRIAHPEEVAS EVLV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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