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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP488349SYWYeast1mgQuote
EP488349SYWE. coli1mgQuote
BP488349SYWBaculovirus200ugQuote
MP488349SYWMammalian Cell200ugQuote

Protein Information

SpeciesShewanella piezotolerans (strain WP3 / JCM 13877)
UniProt IDB8CM41
Gene NameleuC; Locus:swp_2215
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-466
Expression Tag6xHis
Purity>90%
AA SequenceMAKTLYEKVWDAHIVVAAEGEAPIIYVDRHLVHEVTSPQAFSGLEVAGRKMRAPEKTFAT MDHNTSTTSASLDALSPMARTQVQTLERNCKEFGVRLYDIHHKNQGIVHVMGPELGITLP GTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQLKAKTMKIEVRGHVAEGITA KDIVLAIIGKIGMDGGTGYVVEFCGEAIEALTMEGRMTVCNMAIEMGAKAGMIAPDATTA EYLKGREFAPKGDNWQQAIAAWAELKSDADATFDATVVLQASDIAPQLTWGTNPGQVVAI DQFVPNPAEETNSTVRSSIEKALEYVDLSAGTLMTNVGINKVFIGSCTNSRIEDLRAAAV HAKGRQVAEGVKAIVVPGSGLVKEQAEAEGLDKIFLEAGFEWRLPGCSMCLAMNDDKLEA GDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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