Request QuoteCatalog Number: xP492944LPWSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP492944LPWYeast1mgQuote
EP492944LPWE. coli1mgQuote
BP492944LPWBaculovirus200ugQuote
MP492944LPWMammalian Cell200ugQuote

Protein Information

SpeciesListeria monocytogenes serotype 4a (strain HCC23)
UniProt IDB8DBU2
Gene NameleuC; Locus:LMHCC_0571
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-462
Expression Tag6xHis
Purity>90%
AA SequenceMGKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFAT MDHNVPTEDIFNIQDLVAKKQIEALQTNCEEFGVTLADMGSDRQGIVHMVGPETGLTQPG KVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQKPKSMGIEINGKLPKGVYAK DIILHLIATYGVAFGTGYAVEYYGETIRNMSMEERMTICNMAIEGGAKMGMMAPDETTFE YVRGREYAPADMEKAIRDWKTLQTDPDAEYDLHIEMDASILEPYVTWGTNPEMGVPFSKA FPEIKDMNYERAYEYMGLKPGQTAEEIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNN IRALVVPGSRQVRNAAESIGLDKIFIEAGFEWREPGCSMCLGMNPDQVPDGVHCASTSNR NFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAIISGGN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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