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Recombinant Intestinal-type alkaline phosphatase (Iap)

Recombinant Intestinal-type alkaline phosphatase (Iap) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001627MOYeast1mgQuote
EP001627MOE. coli1mgQuote
BP001627MOBaculovirus200ugQuote
MP001627MOMammalian Cell200ugQuote

Protein Information

SpeciesMus musculus (Mouse)
UniProt IDP24822
Gene NameIap; aka: Akp-3, Akp3
Protein NameIntestinal-type alkaline phosphatase
Region Expressed20-528
Expression Tag6xHis
Purity>90%
AA SequenceVIPVEEENPAFWNKKAAEALDAAKKLQPIQTSAKNLIIFLGDGMGVPTVTATRILKGQLE GHLGPETPLAMDRFPYMALSKTYSVDRQVPDSASTATAYLCGVKTNYKTIGLSAAARFDQ CNTTFGNEVFSVMYRAKKAGKSVGVVTTTRVQHASPSGTYVHTVNRNWYGDADMPASALR EGCKDIATQLISNMDINVILGGGRKYMFPAGTPDPEYPNDANETGTRLDGRNLVQEWLSK HQGSQYVWNREQLIQKAQDPSVTYLMGLFEPVDTKFDIQRDPLMDPSLKDMTETAVKVLS RNPKGFYLFVEGGRIDRGHHLGTAYLALTEAVMFDLAIERASQLTSERDTLTIVTADHSH VFSFGGYTLRGTSIFGLAPLNALDGKPYTSILYGNGPGYVGTGERPNVTAAESSGSSYRR QAAVPVKSETHGGEDVAIFARGPQAHLVHGVQEQNYIAHVMASAGCLEPYTDCGLAPPAD ESQTTTTTRQTTITTTTTTTTTTTTPVHN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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