27 units/mg.
Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts).
The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator.
The protein was lyophilized from 50mM Sodium Chloride solution.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Insulin in PBS pH-7.5.
Saccharomyces cerevisiae.
Lyophilized Insulin should be stored at 4oC. Upon reconstitution Insulin should be stored at -20oC to -80oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.