$90.00Catalog Number: PYT614-2mgSize: 2mg

Insulin Human, Yeast

Insulin Human Recombinant, Yeast

Insulin Human Recombinant produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Zinc content was found to be 0.4%. Insulin is purified by proprietary chromatographic techniques.

Accession

P01308

Biological Activity

27 units/mg.
Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts).
The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator.

Formulation

The protein was lyophilized from 50mM Sodium Chloride solution.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Insulin in PBS pH-7.5.

Source

Saccharomyces cerevisiae.

Stability

Lyophilized Insulin should be stored at 4oC. Upon reconstitution Insulin should be stored at -20oC to -80oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Quantity

$90.00

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