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Recombinant D-inositol 3-phosphate glycosyltransferase (mshA)

Recombinant D-inositol 3-phosphate glycosyltransferase (mshA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP515149DQWYeast1mgQuote
EP515149DQWE. coli1mgQuote
BP515149DQWBaculovirus200ugQuote
MP515149DQWMammalian Cell200ugQuote

Protein Information

SpeciesCellulomonas flavigena (strain ATCC 482 / DSM 20109 / NCIB 8073 / NRS 134)
UniProt IDD5UJ42
Gene NamemshA; Locus:Cfla_0653
Protein NameD-inositol 3-phosphate glycosyltransferase
Region Expressed1-443
Expression Tag6xHis
Purity>90%
AA SequenceMTTDRAPRVAMLSVHTSPLDQPGTGDAGGMNVYVLELAHALAARGARVEVFTRATRSDVP ETVVLDGVDAAGRALTADDARDVLLAHDVPPGVTPPVLVHHVPAGPFEALDKNDLPGVLC GMAAGVLRSEAARRPGWYDVVHSHYWLSGQVGAIAAQRWEVPLVHTAHTLAKVKNASLGP GDSAEPSVRVVGEEQVVADADALVASTPVEARELVELYGADPARVHVVEPGVDLERFRPG GPGARDEARRRLGLPTDRPVVLFAGRVQPLKAPDVLVQAVGVLRASGRPVPLLVVLGGPS GRPTAVRELRALAVTLGVDDDVVVRPPAPRDELVSWYRAADLVAMPSRSESFGLVAVEAQ ASGTPVLAADVGGLRTVVEDDVSGRLVPGHDPQVWAEVIADALADAPRRARWAAGARQVA ERYAWTTAADQVLKVYAVAAEPR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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