The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Leu-Ser-Gly.
As measured by its binding ability in a functional ELISA, immobilized IL1F5 at 1 ug/ml (100 ul/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15- 5 ug/ml.
IL1F5 was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to quick spin followed by reconstitution of IL1F5 in PBS to a concentration no less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Although stable at room temperature for 3 weeks, lyophilized IL1F5 should be stored desiccated below -18oC. Upon reconstitution IL1F5 should be stored at 4oC between 2-7 days and for future use below -18oC.
Please avoid multiple freeze-thaw cycles.
Interleukin-1 family member 5, IL-1F5, FIL1 delta, IL-1-related protein 3, IL-1RP3, Interleukin-1 HY1, IL-1HY1, Interleukin-1 delta, IL-1 delta, Interleukin-1 receptor antagonist homolog 1, IL-1ra homolog 1, Interleukin-1-like protein 1, IL-1L1, FIL1D, IL1HY1, IL1L1, IL1RP3, FIL1, IL1F5, IL36RA, MGC29840.
