The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Ile-Pro-Met.
The ED50 range=0.3-1.0 ng/ml corresponding to a specific activity of 1-3.4MIU/mg as determined by the dose-dependant stimulation of the proliferation of BCL-1 cells.
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Interleikin-5 Rat in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Interleukin-5 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL5 Rat should be stored at 4oC between 2-7 days and for future use below -18oC.
Please avoid freeze thaw cycles.
EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, IL-5.