$5,335.20Catalog Number: PYT373-1mgSize: 1mg

IL 9 Mouse

Interleukin-9 Mouse Recombinant

Interleukin-9 Mouse Recombinant produced in E.Coli is a single, non-glycosylated single polypeptide chain containing 127amino acids and having a molecular mass of 14,166 Dalton.
The IL-9 is purified by proprietary chromatographic techniques.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Accession

P15247

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Arg-Cys-Ser.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of human MO7e cells is < 0.5 ng/ml, corresponding to a Specific Activity of 2MIU/mg.

Formulation

Lyophilized from a concentrated (1mg/ml) solution containing no additives.

Protein content

Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-9 as a Reference Standard.

Purity

Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Interleukin 9 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Interleukin-9 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL9 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid multiple freeze-thaw cycles.

Synonyms

P40, HP40, T-cell growth factor p40, IL-9, P40 cytokine.

Quantity

$5,335.20

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