$90.00Catalog Number: PYT350-2ugSize: 2ug

IL 6 Mouse

Interleukin-6 Mouse Recombinant

Interleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton.
The IL-6 is purified by proprietary chromatographic techniques.

Accession

P08505

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.

Biological Activity

The ED50 as determined by the dose-dependant induction of 7TD1 cell proliferation,  is 0.018 ng/ml corresponding to a Specific Activity of 55MIU/mg.

Formulation

The protein was lyophilized without any additives.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Interleukin 6 in 10mM HCl to 0.1-1.0 mg/ml to regain full activity, and can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL6 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid multiple freeze-thaw cycles.

Synonyms

IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor.

Quantity

$90.00

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