APVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM.
The activity was measured in a cell prolifiration assay using B9 cells. The ED50 for this effect is 0.008-0.02ng/ml corresponding to a Specific Activity of 50-125MIU/mg.
Lyophilized from a solution containing Phosphate- Buffered Saline pH-7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Interleukin-6 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Chinese Hamster Ovarian Cells.
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL6 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid multiple freeze-thaw cycles.
IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.
