$3,078.00Catalog Number: PYT722-1mgSize: 1mg

IL 3 Dog

Interleukin-3 Canine Recombinant

Interleukin-3 Canine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 14 kDa. The IL-3 is purified by proprietary chromatographic techniques.

Accession

Q9BDX4

Amino acid sequence

RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 1X PBS pH7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized IL3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized IL3 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL3 should be stored at 4oC between 2-7 days and for future use below -18oC. Please avoid multiple freeze-thaw cycles.

Synonyms

MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.

Quantity

$3,078.00

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