Sterile Filtered White lyophilized (freeze-dried) powder.
MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQGEPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRFVFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS.
The ED50 as determined by the dose-dependant stimulation of mouse D10S cells was found to be less than 2.0 pg/ml, corresponding to a Specific Activity of 5.0 x 108 IU/mg.
Mouse IL-1b was lyophilized from a 0.2um filtered concentrated (1.0mg/ml) solution in PBS, pH 7.5.
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Interleukin-1b in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL1b should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid multiple freeze-thaw cycles.
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
