$148.20Catalog Number: PYT056-5ugSize: 5ug

IL 12 p40 Human, Plant

Interleukin-12 p40 Human Recombinant, Plant

IL-12 p40 human Recombinant produced in Nicotiana benthamiana plant is a glycosilated polypeptide chain containing 312 amino acids, 23-328 aa (molecular formula C1575H2420N424O490S12).
IL-12 p40 is fused to a 6-His-tag at the N-terminal having the total molecular mass of 35.5kDa however as a result of potential glycosylation, the molecular mass could be approximately 40kDa by SDS PAGE under reducing conditions.
IL-12 p40 IS purified by standard chromatographic techniques.

Accession

P29460

Amino acid sequence

HHHHHHIWEL KKDVYVVELD WYPDAPGEMV VLTCDTPEED GITWTLDQSS EVLGSGKTLT IQVKEFGDAG QYTCHKGGEV LSHSLLLLHK KEDGIWSTDI LKDQKEPKNK TFLRCEAKNY SGRFTCWWLT TISTDLTFSV KSSRGSSDPQ GVTCGAATLS AERVRGDNKE YEYSVECQED SACPAAEESL PIEVMVDAVH KLKYENYTSS FFIRDIIKPD PPKNLQLKPL KNSRQVEVSW EYPDTWSTPH SYFSLTFCVQ VQGKSKREKK DRVFTDKTSA TVICRKNASI SVRAQDRYYS SSWSEWASVP CS

Formulation

Lyophilized from 1mg/ml solution in 20 mM PBS buffer pH 7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by Analysis by SDS-PAGE.

Serological Identification

The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electro blotting to a NC membrane and visualized by immune-detection with specific IL12 antibody.

Solubility

It is recommended to reconstitute the lyophilized IL-12 p40 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Nicotiana benthamiana plant

Stability

Lyophilized IL-12 p40 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL-12 p40 should be stored at 4oC between
2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid multiple freeze-thaw cycles.

Synonyms

NKSF2, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), TSF, Edodekin-alpha, IL-12 p40, IL-12B, IL-12 subunit p40, NK cell stimulatory factor chain 2.

Quantity

$148.20

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