$148.20Catalog Number: PYT362-10ugSize: 10ug

IL 12 Human

Interleukin-12 Human Recombinant

Interleukin-12 Human Recombinant produced in CHO is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
The IL-12 is purified by proprietary chromatographic techniques.

Accession

0

Amino acid sequence

The sequence of the first five N-terminal amino acids of IL-12 was determined and found to be Arg-Asn-Leu-Pro-Val at the p35 subunit and Ile-Trp-Glu-Leu-Lys at the p40 subunit.

Biological Activity

IL-12 activity was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. The ED50 for this effect is 0.3-0.5 ng/ml corresponding to a specific activity of 2-3.3MU/mg.

Formulation

IL-12 was lyophilized from a 0.2um filtered solution (0.5mg/ml) in PBS, pH 7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized IL-12 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

CHO (Chinese Hamster Ovarian Cells).

Stability

Lyophilized IL-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL-12 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid multiple freeze-thaw cycles.

Synonyms

NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.

Quantity

$148.20

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