Insulin-Like Growth Factor Binding Protein-5 Human Recombinant
IGFBP5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 253 amino acids and having a molecular mass of 28613 Dalton.
IGFBP5 is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Gly-Ser-Phe.
Biological Activity
The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 ug/ml in the presence of 15ng/ml of Human IGF-II corresponding to a Specific Activity of 66,667IU/mg.
Formulation
IBP-5 was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium Citrate PH 3.0.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IGFBP 5 should be stored at 4oC between 2-7 days and for future use below
-18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
IGFBP-5, IBP-5, IGF-binding protein 5.
