The ED50, was calculated 2 methods 1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be < 30ng/ml corresponding to a Specific Activity of 33,334IU/mg. 2. Type 1 IGF receptor binding assay ED50 was found to be <10ng/ml corresponding to a Specific Activity of 100,000IU/mg.
The protein was lyophilized with no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by SDS-PAGE.
It is recommended to reconstitute the lyophilized IGF1 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized IGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IGFI should be stored at 4oC between 2-7 days and for future use below
-18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Somatomedin C, IGF-I, IGFIA, IGF1.
