The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Gly-Thr-Val.
The specific activity as determined by the cytopathic affect inhibition assay with murine NIH 3T3 cells challenged with EMC virus was < 0.1 ng/ml, corresponding to a specific activity of 10MU/mg.
Lyophilized from a 0.2¦Ìm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 20mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IFN-gamma should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
