Interferon-Alpha 1a Human Recombinant
Interferon-alpha 1a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19.4 kDa.
The Interferon-alpha 1a contains Valine residue at position 114.
The IFN-a 1a is purified by proprietary chromatographic techniques.
Accession
Amino Acid Sequence
CDLPETHSLD NRRTLMLLAQ MSRISPSSCL MDRHDFGFPQ EEFDGNQFQK APAISVLHEL IQQIFNLFTT KDSSAAWDED LLDKFCTELY QQLNDLEACA MQEERVGETP LMNVDSILAV KKYFRRITLY LTEKKYSPCA WEVVRAEIMR SLSLSTNLQE RLRRKE.
Biological Activity
The specific activity as determined in a viral resistance assay viral ressistance assay was found to be 100MIU/ mg.
Formulation
Lyophilized from a (1 mg/ml) solution in containing 1X PBS pH-7.4.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized IFN alpha 1a in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized Interferon alpha 1a although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IFN-alpha 1a should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Interferon-alpha 1a, IFN-a 1a, IFN alpha 1a.
