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Recombinant (R) -2-hydroxyglutaryl-CoA dehydratase subunit alpha

Recombinant (R) -2-hydroxyglutaryl-CoA dehydratase subunit alpha can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP320884AWAYeast1mgQuote
EP320884AWAE. coli1mgQuote
BP320884AWABaculovirus200ugQuote
MP320884AWAMammalian Cell200ugQuote

Protein Information

SpeciesAcidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
UniProt IDP11569
Gene NamehgdA; Locus:Acfer_1815
Protein Name (R) -2-hydroxyglutaryl-CoA dehydratase subunit alpha
Region Expressed2-477
Expression Tag6xHis
Purity>90%
AA SequencePKTVSPGVQALRDVVEKVYRELREAKERGEKVGWSSSKFPCELAESFGLHVGYPENQAAG IAANRDGEVMCQAAEDIGYDNDICGYARISLAYAAGFRGANKMDKDGNYVINPHSGKQMK DANGKKVFDADGKPVIDPKTLKPFATTDNIYEIAALPEGEEKTRRQNALHKYRQMTMPMP DFVLCCNNICNCMTKWYEDIARRHNIPLIMIDVPYNEFDHVNEANVKYIRSQLDTAIRQM EEITGKKFDEDKFEQCCQNANRTAKAWLKVCDYLQYKPAPFNGFDLFNHMADVVTARGRV EAAEAFELLAKELEQHVKEGTTTAPFKEQHRIMFEGIPCWPKLPNLFKPLKANGLNITGV VYAPAFGFVYNNLDELVKAYCKAPNSVSIEQGVAWREGLIRDNKVDGVLVHYNRSCKPWS GYMPEMQRRFTKDMGIPTAGFDGDQADPRNFNAAQYETRVQGLVEAMEANDEKKGK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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