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Recombinant 3- (3-hydroxy-phenyl) propionate/3-hydroxycinnamic acid hydroxylase

Recombinant 3- (3-hydroxy-phenyl) propionate/3-hydroxycinnamic acid hydroxylase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP302865ENVYeast1mgQuote
EP302865ENVE. coli1mgQuote
BP302865ENVBaculovirus200ugQuote
MP302865ENVMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12)
UniProt IDP77397
Gene NamemhpA; Locus:b0347, JW0338
Protein Name3- (3-hydroxy-phenyl) propionate/3-hydroxycinnamic acid hydroxylase
Region Expressed1-554
Expression Tag6xHis
Purity>90%
AA SequenceMAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGID DEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVD AVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEGQREIVKAQWLVACDGGASFV RRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIM PVWQGQGYNSGMRDAFNLAWKLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVL APPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYYGGALMREGEAKHSPVGKMFI QPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALGTRFIQVVPEVQIH TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMT LTRPDADVSVEKVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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