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Recombinant 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD)

Recombinant 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP342870BNVYeast1mgQuote
EP342870BNVE. coli1mgQuote
BP342870BNVBaculovirus200ugQuote
MP342870BNVMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia xenovorans (strain LB400)
UniProt IDP47229
Gene NamebphD; Locus:Bxeno_C1120; ORFs:Bxe_C1186
Protein Name2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
Region Expressed1-286
Expression Tag6xHis
Purity>90%
AA SequenceMTALTESSTSKFVKINEKGFSDFNIHYNEAGNGETVIMLHGGGPGAGGWSNYYRNVGPFV DAGYRVILKDSPGFNKSDAVVMDEQRGLVNARAVKGLMDALDIDRAHLVGNSMGGATALN FALEYPDRIGKLILMGPGGLGPSMFAPMPMEGIKLLFKLYAEPSYETLKQMLQVFLYDQS LITEELLQGRWEAIQRQPEHLKNFLISAQKAPLSTWDVTARLGEIKAKTFITWGRDDRFV PLDHGLKLLWNIDDARLHVFSKCGHWAQWEHADEFNRLVIDFLRHA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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