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Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG)

Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP302601EZGYeast1mgQuote
EP302601EZGE. coli1mgQuote
BP302601EZGBaculovirus200ugQuote
MP302601EZGMammalian Cell200ugQuote

Protein Information

SpeciesProvidencia stuartii
UniProt IDP72241
Gene NameispG; aka: aarC
Protein Name4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Region Expressed1-365
Expression Tag6xHis
Purity>90%
AA SequenceMHNESPIKRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVRQIQSLERVGVDIVR VSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIAMKVAEYGVDCLRINPGNIGSEERIRQ VVDSARHHNIPIRIGVNGGSLEKDIQEKYGEPTPEALVESAMRHVDILDRLNFDQFKVSV KASDVFLAVGSYRLLAQKIDQPLHLGITEAGGARSGSVKSAIGLGMLLAEGIGDTLRISL AADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV SIIGCVVNGPGEAEVSTLGVAGAKTKSGFYEDGVRKKERFDNDNIIDQLEAKIRAKAAML DEITV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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