Request QuoteCatalog Number: xP514798ESQSize: 0.2-1mg

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Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)

Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514798ESQYeast1mgQuote
EP514798ESQE. coli1mgQuote
BP514798ESQBaculovirus200ugQuote
MP514798ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DF01
Gene NameispH; Locus:PC1_3650
Protein Name4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Region Expressed1-316
Expression Tag6xHis
Purity>90%
AA SequenceMQILLANPRGFCAGVDRAISIVERALELFGTPIYVRHEVVHNRYVVNGLRERGAIFIEQI EDVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKVHMEVARASKKGIEAILIG HAGHPEVEGTMGQYSNADGGMYLVESPEDVWQLQVKDESNLCFMTQTTLSVDDTYAVIDA LRERFPQIVGPRKDDICYATTNRQEAVRHLSDKADVVFVVGSKNSSNSNRLAELAQRAGK AAYLIDSAEDIQESWVEGASHVGVTAGASAPDILVQQVIQRLKALGGKVAVEMQGREENI VFEVPKELRVEVKQVD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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