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Request QuoteCatalog Number: xP479158SWSSize: 0.2-1mg

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Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)

Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP479158SWSYeast1mgQuote
EP479158SWSE. coli1mgQuote
BP479158SWSBaculovirus200ugQuote
MP479158SWSMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella newport (strain SL254)
UniProt IDB4T6H5
Gene NameispH; Locus:SNSL254_A0053
Protein Name4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Region Expressed1-316
Expression Tag6xHis
Purity>90%
AA SequenceMQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRYVVDSLRKRGAIFIEQI SEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKVHMEVARASRRGEESILIG HAGHPEVEGTMGQYSNPEGGMYLVESPEDVWTLNVKNEGKLSFMTQTTLSVDDTSDVIDA LRKRFPKIVGPRKDDICYATTNRQEAVRALAEQADVVLVVGSKNSSNSNRLAELAQRMGR TAFLIDDAADIQEAWVKEAACVGVTAGASAPDILVQNVIARLREFGGGEAVTLEGREENI VFEVPKELRVDVREVE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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