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Recombinant 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX)

Recombinant 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP499078BWLYeast1mgQuote
EP499078BWLE. coli1mgQuote
BP499078BWLBaculovirus200ugQuote
MP499078BWLMammalian Cell200ugQuote

Protein Information

SpeciesBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
UniProt IDC0ZL41
Gene NamemtnX; Locus:BBR47_48910
Protein Name2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
Region Expressed1-222
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLVLFCDFDGTITEKDNIVAIVRKFAPPEWEALTEQILSQKISVQEGVGKLFQLLPS SLRQDIIDFIVHEATIRPGFAEFVSYCREEGIELLITSGGIDFFLEPILAPFDLADVPIY CNGSDFSGERITITWPNACDEHCTNGCGMCKTTIIRRYDPATHFRIVIGDSITDLAGAKI ADYVIARSFLADKAEELQLPHSTFATFHDVIRILQQVQQEVV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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