HHHHHHGSAKTIAYDEEARRGLERGLNALADAVKV
TLGPKGRNVVLEKKWGAPTITNDGVSIAKEIELEDPY
EKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVRE
GLRNVAAGANPLGLKRGIEAVEKVTETLLKGAKEVET
KEQIAATAAISAGDQSIGDLIAEAMDKVGNEGVITV
EESNTFGLQLELTEGMRFDKGYISGYFVTDPERQEAV
LEDPYILLVSSKVSTVKDLLPLLEKVIGAGKPLLIIEDVEGE
ALSTLVVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAIL
TGGQVISEEVGLTLENADLSLLGKARKVVVTKDETTIVE
GAGDTDAIAGRVAQIRQEIENSDSDYDREKLQERLAKL
AGGVAVKAGAATEVELKERKHRIEDAVRNAKAAVEEGIV
AGGGVTLLQAAPTLDELKLEGDEATGANIVKVALEAPL
KQIAFNSGLEPGVVAEKVRNLPAGHGLNAQTGVYEDLLA
AGVADPVKVTRSALQNASIAGLFLTTEAVVADKPEKEKASVPGGGDMGGMDF.
The HSP65 protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Na-phosphate pH-7.4, 130mM NaCl and 2.5mM KCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized HSP-65 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized HSP65 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution HSP65 should be stored at 4oC between 2-7 days and for future use below
-18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Protein Cpn60-2, groEL protein-2, 65 kDa antigen, Heat shock protein 65, Cell wall protein A, Antigen A, groL2, groEL-2.
