HSP27 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.

P2A5AT.

Mouse IgG1 heavy chain and κ light chain.

Anti-human HSP27 mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HSP27 amino acids 1-205 purified from E. coli.

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

HSP27 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

12 months at -20^oC. 1 month at 4^oC.

For periods up to 1 month store at 4^oC, for longer periods of time, store at -20^oC. Prevent freeze thaw cycles.

HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.

Mouse Anti Human Monoclonal.