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Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA)

Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514356ENUYeast1mgQuote
EP514356ENUE. coli1mgQuote
BP514356ENUBaculovirus200ugQuote
MP514356ENUMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12 / MC4100 / BW2952)
UniProt IDC4ZQE5
Gene NameruvA; Locus:BWG_1675
Protein NameHolliday junction ATP-dependent DNA helicase RuvA
Region Expressed1-203
Expression Tag6xHis
Purity>90%
AA SequenceMIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQEAIVFTHFVVREDAQLL YGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREEVGALVKLPGIGKKT AERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQEAVAALVALGYKPQEASRM VSKIARPDASSETLIREALRAAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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