$185.00Catalog Number: VHIV001-10ugSize: 10ug

HIV-1 Protease

HIV-1 Protease Recombinant

HIV-1 protease is an active homodimer having a molecular mass of 21.6kDa (each monomer of 99 amino acids is 10.8kDa).

Amino Acid Sequence

PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMNLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF

Formulation

The HIV-1 Protease filtered (0.4?m) solution (0.27mg/1ml) is formulated in 20mM Tris, 20mM MES, 200mM NaCl, 1mM EDTA, 10% (v/v) glycerol and 0.05% 2-mercaptoethanol, pH 6.5.

Kinetic parameters

Km=15.1uM, Kcat = 30s-1, Kcat/Km= 1981 mM-1s-1 with peptide substrate KARVF (NO2)VRKA (F(NO2) . . . p-nitrophenylalanine).

Physical Appearance

Sterile filtered colorless clear solution.

Purity

Greater than 95% as determined by densitometric image analysis.

Source

E. coli

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

Quantity

$185.00

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