HIV-1 nef Recombinant
The E. coli derived 20 kDa recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 nef immunodominant regions, 3-190 amino acids.
The HIV-1 nef is fused to beta-galactosidase (114 kDa) at the N-terminus.
Amino Acid Sequence
kwskssv igwptvrerm rraepaadgv gaasqdlekh gaitssntaa tnadcawleaqeeeevgfpv tpqvplrpmt ykaavdlshf lkekgglegl ihsqrrqdil dlwiyhtqgyfpdwqnytpg pgirypltfg wcyklvpvep ekleeankge ntsllhpvsl hgmddperevlewrfdsrla.
Applications
HIV-1 nef antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
Formulation
HIV-1 nef protein solution in 8M urea, 20mM Tris-HCl pH 8.0, 10mM b-mercaptoethanol.
Physical Appearance
Sterile filtered colorless clear solution.
Purity
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.
Source
Specificity
HIV-1 nef is immunoreactive with all sera of HIV-1 infected individuals.
Storage
HIV-1 nef although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
