HIV-1 Envelope Recombinant
HIV-1 envelope is an E. coli-derived recombinant protein that composes all of the reported immunogenic determinants found in gp41 and a small portion of gp120.The gene encoding this fusion protein was synthesized using codons optimized for E. coli expression and doesn??t represent a linear HIV-1 envelope sequence. HIV-1 is a non-glycosylated, 233 amino acid polypeptide chain, having a molecular mass of 27275.88 dalton and pI=9.68.HIV-1 envelope protein spans the C-Terminus of gp120 and most of gp41.Superior diagnostic reagent for HIV-1 and HIV type-O detection. Detects all HIV-1 and HIV-type O infected individuals responding to envelope proteins.
Applications
HIV-1 Envelope antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
Formulation
The HIV-1 contains 0.5X PBS & 6M urea.
Physical Appearance
Sterile filtered colorless clear solution.
Purity
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.
Source
Specificity
Immunoreactive with all sera of HIV-1 and HIV-type O infected individuals and with 60-80% of HIV-2 infected individuals.
Storage
HIV-1 Envelope although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
