The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5 and is typically 10-50ng/ml.
The HGF was lyophilized from 1mg/ml in 1xPBS.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95% as obsereved by SDS-PAGE.
It is recommended to reconstitute the lyophilized HGF in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution HGF should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF.
