$185.00Catalog Number: VHCV004-10ugSize: 10ug

HCV NS5A

Hepatitis C Virus NS5A Recombinant

Recombinant HCV NS5A produced in E. coli is a single polypeptide chain containing 118 amino acids (aa 2210-2306) and having a molecular mass of 13.4kDa. Recombinant HCV NS5A is fused to a 21 amino acid His-tag at N-terminus and purified by proprietary chromatographic techniques.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSLKATCTAN HDSPDAELIE ANLLWRQEMG GNITRVESEN KVVILDSFDP LVAEEDEREV SVPAEILRKS RRFARALPVW ARPDYNPPLV ETWKKPDY.

Formulation

The HCV NS5A protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol and 1mM DTT.

Physical Appearance

Sterile filtered colorless solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Source

E. coli

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Quantity

$185.00

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