Hepatitis C Virus NS3 (1450-1643 a.a.) Recombinant
The E. coli derived 22kDa recombinant protein contains the HCV NS3 genotype 1b immunodominant regions, amino acids 1450-1643. The protein is fused with b-galactosidase (114 kDa) at N-terminus, pI 5.43.
Applications
HCV NS3 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Formulation
20mM Tris-Hcl pH-8, 8M urea &10mM B-ME.
Purification Method
HCV NS3 protein was purified by proprietary chromatographic technique.
Purity
HCV NS3 protein is >95% pure as determined by 10% PAGE (coomassie staining).
Specificity
Immunoreactive with sera of HCV-infected individuals.
Storage
HCV NS3 although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
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