Hepatitis B Virus e-Antigen Recombinant
Recombinant hepatitis B virus ??e?? antigen produced in E. coli, is a 139 a. a. protein. The HBeAg sequence is derived from HBV adw2 and fused to 6xHis Tag at C-terminus.
Amino Acid Sequence
SKLCLGWLWG MDIDPYKEFG ATVELLSFLP SDFFPSVRDL LDTASALYRE ALESPEHCSP HHTALRQAIL CWGELMTLAT WVGNNLEDPA SRDLVVNYVN TNVGLKIRQL LWFHISCLTF GRETVLEYLV SFGVWIRTPP AYRPPNAPIL STLPETTVVR RRDRGRSPRR RTPSPRRRRS PSPRRRRSQS RESQC
Applications
Antigen in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.
Formulation
Phosphate buffered saline, pH 7.4.
Purification Method
Purified by proprietary chromatographic technique.
Purity
Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Source
Specificity
Immunoreactive with sera HBV-infected individuals.
Storage
HBeAg although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
