GPC4 antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.
Recommended starting dilution is 1:500.
Mouse IgG1 heavy chain and κ light chain.
Anti-human GPC4 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GPC4 amino acids 401-529 purified from E. coli.
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
GPC4 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
12 months at -20oC. 1 month at 4oC.
For periods up to 1 month store at 4oC, for longer periods of time, store at -20oC. Prevent freeze thaw cycles.
Glypican-4, K-glypican, GPC4.
Mouse Anti Human Monoclonal.