The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.
The ED50 range = 0.001-0.01ng/ml corresponding to a specific activity of 100-1,000MIU/mg, determined by the dose dependent proliferation of murine MC/9 cells.
GM-CSF Rat was lyophilized with no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
GMCSF quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.966 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated the PC GENE computer analysis program of protein sequences (telliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.
Greater than 95.0% as determined by:
(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution GMCSF should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.
