$90.00Catalog Number: PYT724-2ugSize: 2ug

GM CSF K9

Granulocyte Macrophage-Colony Stimulating Factor Canine Recombinant

GMCSF k9 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GM-CSF is purified by proprietary chromatographic techniques.

Accession

P48749

Amino Acid Sequence

APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE
GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN
INFKSFKENL KDFLFNIPFD CWKPVKK.

Biological Activity

The ED50 as calculated by the dose-dependent stimulation of the proliferation of human TF1 erythroleukemic cells is typically 1-4 ng/ml.

Formulation

GMCSF was lyophilized after extensive dialysis against 1xPBS pH 7.4.

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 96.0% as determined by
1. Analysis by RP-HPLC.
2. Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized GMCSF in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution GMCSF should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.

Quantity

$90.00

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