Request QuoteCatalog Number: xP001446YAWSize: 0.2-1mg

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Recombinant Glycogen debranching enzyme (glgX)

Recombinant Glycogen debranching enzyme (glgX) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001446YAWYeast1mgQuote
EP001446YAWE. coli1mgQuote
BP001446YAWBaculovirus200ugQuote
MP001446YAWMammalian Cell200ugQuote

Protein Information

SpeciesYersinia pseudotuberculosis serotype IB (strain PB1/+)
UniProt IDB2K6G0
Gene NameglgX; Locus:YPTS_3983
Protein NameGlycogen debranching enzyme
Region Expressed1-662
Expression Tag6xHis
Purity>90%
AA SequenceMAVLTHGSPTPSGAYFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGY LPGGKPGQRYGYRVSGPFDPSRGHRFNPHKLLIDPRARALEGKVGDDPRFTGGVSQPDVR DSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVVYEAHVRGLTQLHPDIPPELRGTYAALA HPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI SPLRELRDAVKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDN ITGCGNALRLSHPYVTQWVIDCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALA ADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRDAMRGFWLRGEVPRGTFAQHF AASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWL DWGSADRALMTFTADLIRLRQQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQ QLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSDHAERLTTWRGSAHTICVLIK RD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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