Request QuoteCatalog Number: xP001446YAXSize: 0.2-1mg

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Recombinant Glycogen debranching enzyme (glgX)

Recombinant Glycogen debranching enzyme (glgX) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001446YAXYeast1mgQuote
EP001446YAXE. coli1mgQuote
BP001446YAXBaculovirus200ugQuote
MP001446YAXMammalian Cell200ugQuote

Protein Information

SpeciesYersinia pseudotuberculosis serotype O:3 (strain YPIII)
UniProt IDB1JHX8
Gene NameglgX; Locus:YPK_0148
Protein NameGlycogen debranching enzyme
Region Expressed1-662
Expression Tag6xHis
Purity>90%
AA SequenceMAVLTHGSPTPSGAYFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGY LPGGKPGQRYGYRVSGPFDPSRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVR DSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRGLTQLHPDIPPELRGTYAALA HPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI SPLRELRDAVKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDN ITGCGNALRLSHPYVTQWVIDCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALA ADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRDAMRGFWLRGEVPRGTFAQHF AASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWL DWGSADRALMTFTADLIRLRQQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQ QLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSDHAERLTTWRGSAHTICVLIK RD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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