The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Thr-Ile.
HGH-20K was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 10.
Sterile Filtered White lyophilized (freeze-dried) powder.
HGH-20K quantitation was carried out by two independent methods::
1. UV spectroscopy at 280 nm using the absorbency value of 0.735 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH-20K as a Reference Standard.
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Growth Hormone Pituitary in 0.4% NaHCO3adjusted to pH 10, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Pituitary Growth Hormone-20K although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution and filter sterilization GH-20K can be stored at 4oC for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.
