$148.20Catalog Number: MAB005-20ugSize: 20ug

GAPDH Antibody

Glyceraldehyde-3-Phosphate Dehydrogenase, Mouse Anti Human
Monoclonal Antibody

Monoclonal antibodies provide the best target specificity and affinity. It is also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.

Applications

GAPDH antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 10,000.
Recommended starting dilution is 1:1,000.

Clone

PAT8G4AT.

Ig Subclass

Mouse IgG2b heavy chain and κ light chain.

Immunogen

Anti-human GAPDH mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GAPDH amino acids 1-335 purified from E. coli.

Protein formulation

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Purification Method

GAPDH antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Stability / Shelf Life

12 months at -20oC. 1 month at 4oC.

Storage Procedures

For periods up to 1 month store at 4oC, for longer periods of time, store at -20oC. Prevent freeze thaw cycles.

Synonyms

G3PD, GAPD, MGC88685, GAPDH, Glyceraldehyde-3-Phosphate Dehydrogenase.

Type

Mouse Anti Human Monoclonal.

Quantity

$148.20

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