MYPNASPLLG SSWGGLIHLY TATARNSYHL QIHKNGHVDG APHQTIYSAL MIRSEDAGFV VITGVMSRRY LCMDFRGNIF GSHYFDPENC RFQHQTLENG YDVYHSPQYH FLVSLGRAKR AFLPGMNPPP YSQFLSRRNE IPLIHFNTPI
PRRHTRSAED DSERDPLNVL KPRARMTPAP ASCSQELPSA EDNSPMASDP LGVVRGGRVN THAGGTGPEG CRPFAKFI.
The biological activity of FGF-23 was measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.05-0.5ug/ml in the presence of 5ug/ml of Recombinant Mouse Klotho and 10 ug/ml of heparin.
The FGF-23 protein (0.5mg/ml) was lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.
Sterile Filtered white lyophilized powder.
Greater than 95.0% as determined by SDS-PAGE.
It is recommended to reconstitute the lyophilized FGF-23 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized FGF-23 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution FGF-23 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Tumor-derived hypophosphatemia-inducing factor, HYPF, ADHR, HPDR2, PHPTC, FGF23, FGF-23, Fibroblast Growth Factor-23.
