Fibroblast Growth Factor-Basic Bovine Recombinant
FGF-2 Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. The Fibroblast Growth Factor 2 is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.
Biological Activity
The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be <0.1 ng/ml, corresponding to a specific activity of 10MUnits/mg.
Formulation
The FGF-b Bovine was lyophilized from a concentrated (1mg/ml) sterile solution containing 1%HSA.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of FGF-2 Bovine as a Reference Standard.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-2 Bovine Recombinant in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized Fibroblast Growth Factor 2 Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution FGF-b Bovine Recombinant should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.
Synonyms
HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.
