Fibroblast Growth Factor-21 Human Recombinant
Fibroblast Growth Factor -21 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and an N-terminal Methionin (bold), having a molecular weight of 19.7 kDa (calculated). The amino acid sequence of the recombinant human FGF21 is 100% homologous to the amino acid sequence of the human FGF21 without signal sequence.
The FGF-21 is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
MHPIPDS SPLLQFGGQV RQRYLYTDDA QQTEAHLEIR EDGTVGGAAD QSPESLLQLK ALKPGVIQIL GVKTSRFLCQ RPDGALYGSL HFDPEACSFR ELLLEDGYNV YQSEAHGLPL HLPGNKSPHR
DPAPRGPARFLPLPGLPPAP PEPPGILAPQ PPDVGSSDPL SMVGPSQGRS PSYAS.
Formulation
Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 20mM TRIS, 20mM NaCl, pH 7.5.
Physical Appearance
Sterile Filtered white lyophilized powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-21 Human Recombinant sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized FGF-21 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Fibroblast Growth Factor 21 should be stored at
4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Fibroblast growth factor 21, FGF-21.
