Fibroblast Growth Factor-21 Human Recombinant, His Tag
Fibroblast Growth Factor -21 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 202 amino acids (29-209) and having a molecular mass of 21.6 kDa.
The FGF-21 is fused to 20 amino acid His Tga at N-terminus and purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVKTSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS.
Biological Activity
Measured in a cell proliferation assay using NIH-3T3 cells. The ED50 for this effect is < 12ng/ml, which corresponds to > 83,000 units/ml.
Formulation
The FGF-21 His tag protein solution in 20mM Tris-HCL buffer pH-8 and 10% glycerol.
Physical Appearance
Sterile Filtered colorless clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Source
Stability
Store at 4oC if entire vial will be used within 2-4 weeks.
Store, frozen at -20oC for longer periods of time.
Please avoid freeze thaw cycles.
Synonyms
Fibroblast growth factor 21, FGF-21.
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