Fibroblast Growth Factor-21 Bovine Recombinant
Fibroblast Growth Factor -21 Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 182 amino acids, having a molecular weight of 19.5 kDa.
The FGF-21 is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-His-Pro-Ile-Pro.
Formulation
The protein was lyophilized from a concentrated (0.8 mg/ml) solution with 0.4 mg/ml of NaHCO3, pH 8.
Physical Appearance
Sterile Filtered white lyophilized powder.
Protein content
Bovine FGF-21 quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of FGF-21 Recombinant as a Reference Standard.
Purity
Greater than 98.0% as determined by:
(a) Analysis by Gel Filtration.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Bovine FGF-21 in sterile water or 0.4% NaHCO3, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Source
Stability
Lyophilized FGF-21 Bovine Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Fibroblast growth factor 21, FGF-21, FGF21.
